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Title: Determination of CD4+ T- Lymphocytes in Healthy Children of Kathmandu
Authors: Karn, Sapana
Bhattarai, Manjula
Rauniyar, Ramanuj
Adhikari, Anurag
Karna, Pratik
Upadhyay, Bishnu Prasad
gupta, Birendra prasad
Citation: KarnS., BhattaraiM., RauniyarR., AdhikariA., KarnaP., UpadhyayB. P., & guptaB. prasad. (2018). Determination of CD4+ T- Lymphocytes in Healthy Children of Kathmandu. Journal of Nepal Health Research Council, 16(3), 325-329.
Issue Date: 2018
Publisher: Nepal Health Research Council
Article Type: Original Article
Keywords: CD4+ T lymphocyte
Series/Report no.: Jul-Sep 2018;1068
Abstract: Abstract Background: The cluster differentiation (CD) of T-cell is the good marker for the immunological competence study. Nepal does not have a reference value for CD4+ T cell count and percentage for children, which severely limits the prospect of pediatric prognosis. Methods: This cross-sectional study was conducted in Kathmandu valley where total 207 children of age 0-14 year age group were recruited in this study. We analyzed 50 cord blood and 157 peripheral blood samples in order to calculate the absolute count of CD4+ T lymphocyte using Fluorescence-activated cell sorting methodology. Results: The reference range for absolute CD4+ T cell count was found to be 634-4040 cells/µL(mean1470; median: 1335 and 95% CI [1322-1617]) for male children and 491-2922 cells/µL (mean: 1443 median: 1326 and95% CI [1298-1588]) for the female children.We also observed elevated CD4 to the CD3 ratio in younger children (0.67 from cord blood Vs 0.53 from 10-14yr) compared to older ones. Conclusions: The observed CD4+ T cell counts among healthy children of Kathmandu highlights the gender differences skewed for male as well the need of defining specific reference values for other lymphocyte subsets as well in a country like Nepal which has a population with diverse genetic and socio-cultural parameters. Keywords: CD4+ T lymphocyte; children; HIV; immunophenotyping; Kathmandu; Nepal.
Description: Original Article
ISSN: Print ISSN: 1727-5482; Online ISSN: 1999-6217
Appears in Collections:Vol. 16 No. 3 Issue 40 Jul - Sep 2018

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