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Title: Molecular Epidemiology and Antigenic Characterization of Seasonal Influenza Viruses Circulating in Nepal
Authors: Upadhyay, Bishnu Prasad
Ghimire, Prakash
Tashiro, Masato
Banjara, Megha Raj
Citation: UpadhyayB. P., GhimireP., TashiroM., & BanjaraM. R. (2017). Molecular Epidemiology and Antigenic Characterization of Seasonal Influenza Viruses Circulating in Nepal. Journal of Nepal Health Research Council, 15(1).
Issue Date: 2017
Publisher: Nepal Health Research Council
Keywords: Characterization
Influenza virus
Series/Report no.: Jan-April, 2017;970
Abstract: Abstract Background: Influenza is one of the public health burdens in Nepal and its epidemiology is not clearly understood. The objective of this study was to explore the molecular epidemiology and the antigenic characteristics of the circulating influenza viruses in Nepal. Methods: A total of 1495 throat swab specimens were collected from January to December, 2014. Real time PCR assay was used for identification of influenza virus types and subtypes. Ten percent of the positive specimens were randomly selected and inoculated onto Madin-Darby Canine Kidney Epithelial cells (MDCK) for influenza virus isolation. All viruses were characterized by the hemagglutination inhibition (HI) assay. Results: Influenza viruses were detected in 421/1495 (28.2%) specimens. Among positive cases, influenza A virus was detected in 301/421 (71.5%); of which 120 (39.9%) were influenza A/H1N1 pdm09 and 181 (60.1%) were influenza A/H3 subtype. Influenza B viruses were detected in 119/421 (28.3%) specimens. Influenza A/H1N1 pdm09, A/H3 and B viruses isolated in Nepal were antigenically similar to the vaccine strain influenza A/California/07/2009(H1N1pdm09), A/Texas/50/2012(H3N2), A/New York/39/2012(H3N2) and B/Massachusetts/2/2012, respectively. Conclusions: Influenza viruses were reported year-round in different geographical regions of Nepal which was similar to other tropical countries. The circulating influenza virus type and subtypes of Nepal were similar to vaccine candidate virus which could be prevented by currently used influenza vaccine. Keywords: Characterization; epidemiology; influenza virus; Nepal.
Description: Original Article
ISSN: Print ISSN: 1727-5482; Online ISSN: 1999-6217
Appears in Collections:Vol. 15 No. 1 Issue 35 Jan-Apr 2017

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