Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14356/1542
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dc.contributor.authorLekhak, Binod-
dc.contributor.authorSingh, Anjana-
dc.contributor.authorBhatta, Dwij Raj-
dc.date.accessioned2023-05-16T05:18:54Z-
dc.date.available2023-05-16T05:18:54Z-
dc.date.issued2018-
dc.identifier.citationLekhakB., SinghA., & BhattaD. R. (2018). Antibacterial and Antifungal Property of Actinomycetes Isolates from Soil and Water of Nepal. Journal of Nepal Health Research Council, 16(2), 136-139. https://doi.org/10.33314/jnhrc.v16i2.1227en_US
dc.identifier.issnPrint ISSN: 1727-5482; Online ISSN: 1999-6217-
dc.identifier.urihttp://103.69.126.140:8080/handle/20.500.14356/1542-
dc.descriptionOriginal Articleen_US
dc.description.abstractAbstract Background: Human pathogens are rapidly acquiring resistance to antibiotics leading to treatment failure. We carried out this study to isolate and screen actinomycetes strains that have potential to kill bacterial and fungal pathogens. Methods: In this descriptive study 288 soil and water samples were processed by standard microbiological techniques at Central Department of Microbiology,Tribhuvan University from 2013 to 2015. Screened actinomycetes were cultivated for bioactive metabolite production and minimum inhibitory concentration (MIC) of metabolites were determined against bacterial pathogens including multidrug resistant bacteria and fungi. Results: One hundred twenty isolates having antimicrobial property were screened. Out of them, four most potent strains, Nocardiopsis prasina, Streptomyces violarus, Streptomyces krainskii and Streptomyces tsusimaensis were identified all having both antibacterial and anti-fungal property.Highest zone of inhibition (ZOI)was given by N. prasina against Candida albicans(41.33 ±1.15mm) and among bacteria, maximum ZOI was against Acinetobacter baumannii(31.33±3.05mm). MIC value of metabolite of N.prasina was 0.125mg/ml for E.coli and C. albicans. It was 2.5 mg/ml each for methicillin resistant Staphylococcus aureus (MRSA), A. baumannii and Salmonella Typhi and 0.625 mg/ml for Bacillus Subtilis. Conclusions: Bioactive metabolite producing actinomycetes were recovered from soil and tested against human pathogenic bacteria and fungiand found to have antibacterial and antifungal property. Keywords: Actinomycetes; bioactive metabolite; MIC; zone of inhibition.en_US
dc.language.isoenen_US
dc.publisherNepal Health Research Councilen_US
dc.relation.ispartofseriesApr-June, 2018;1227-
dc.subjectActinomycetesen_US
dc.subjectBioactive metaboliteen_US
dc.subjectMICen_US
dc.subjectZone of inhibitionen_US
dc.titleAntibacterial and Antifungal Property of Actinomycetes Isolates from Soil and Water of Nepalen_US
dc.typeJournal Articleen_US
local.journal.categoryOriginal Article-
Appears in Collections:Vol. 16 No. 2 Issue 39 Apr-Jun 2018

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